Part:BBa_K2617006

GroEL

Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 880
  Illegal AgeI site found at 1341
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.

Fig. 1 The measurement of optical density (A600) by periodically taking samples.

References

1.Zingaro, K. A., & Papoutsakis, E. T. (2013). GroESL overexpression imparts Escherichia coli tolerance to i-, n-, and 2-butanol, 1, 2, 4-butanetriol and ethanol with complex and unpredictable patterns. Metabolic engineering, 15, 196-205.