Reporter

Part:BBa_K2610044

Designed by: Charlotte de Ceuninck van Capelle   Group: iGEM18_Leiden   (2018-10-10)
Revision as of 12:18, 10 October 2018 by Chardeceuninck (Talk | contribs)


pSoxS-mCherry-pMicF-GFP

This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_K180008) as well as the regulatory part promoter MicF (BBa_K2610007) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.

The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1976


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Parameters
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