Composite

Part:BBa_K2666004

Designed by: Léa Meneu   Group: iGEM18_Montpellier   (2018-09-29)
Revision as of 07:29, 29 September 2018 by Registry (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


VHH LaM-4 with signal peptid YncM with a L. jensenii specific strong promoter

The Montpellier iGEM team 2018 designed a construction that produce and secrete LaM4, a nanobody against RFP and mcherry in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is B. subtilis specific [2]. Figure 1 illustrates the detailed design.

Figure 1 : Construct design. The sequence that encode scFv LaM-4 with a SP YncM and produce by a strong promoter RpsU.

LaM4 is used as a control [3]. It is an antibody against RFP & mcherry. This control allow us to check the efficiency of the promoter and the signal peptid.

Proof of function WB ELISA Test

Sequence and feature seq on benchling



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 62
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None