Composite

Part:BBa_K2666000

Designed by: Léa Meneu   Group: iGEM18_Montpellier   (2018-09-27)
Revision as of 08:10, 29 September 2018 by Leameneu (Talk | contribs)

The Montpellier iGEM team 2018 designed a construction that produce and secrete LaM4, a nanobody against RFP and mcherry in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is L. jensenii specific [2]. Figure 1 illustrates the detailed design.


Figure 1 : Construct design. The sequence that encode scFv LaM-4 with a SP CsbA and produce by a strong promoter RpsU.

LaM4 is used as a control [3]. It is an antibody against RFP & mcherry. This control allow us to check the efficiency of the promoter and the signal peptid.

[edit]
Categories
Parameters
None