DNA

Part:BBa_K2614000:Design

Designed by: Nicole Banholzer, Rick Seifert   Group: iGEM18_JMU_Wuerzburg   (2018-10-09)
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template as positive control for a qPCR that detects Plasmodium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplified the template by a PCR and digested with EcoRI an PstI. Subsequently we digested the pSB1C3 plasmid with EcoRI and PstI and ligated the digested Plasmid with our digested template.


Source

The template was syntesized by a company.

References