![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2614000
Designed by: Nicole Banholzer, Rick Seifert Group: iGEM18_JMU_Wuerzburg (2018-10-09)
template as positive control for a qPCR that detects Plasmodium
We developed a primer/probe set for a qPCR, which detects the human patogens of the genus Plasmodium. This part can be used as positive control for our qPCR. If you like to run this qPCR you need one sense primer (CTGGCTAAACTTCCCAATGA), one antisense primer (GTCTATATTCATGTTTGATTGACCTT) and a dual labeled Probe ([6FAM]CTTcCAaATaGAtTTCGCAGAA[BHQ1] (lowercase letter = LNA)). The easiest way is synthesizing this oligos by a company.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |