Composite

Part:BBa_K2669003

Designed by: Matilda Brink   Group: iGEM18_Uppsala   (2018-10-02)
Revision as of 16:26, 7 October 2018 by MatildaBrink (Talk | contribs)


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator

Composite part containing codon optimized original native AmilCP BBa_K2669002, RBS BBa_J34801, constitutive promotor BBa_J23119 and a double terminator BBa_B0014. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture.

Part can be used as a reporter and is expressed constitutively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
Parameters
abs588
chassisE.coli DH5-alpha
colorBlue
controlConstitutive