Plasmid

Part:BBa_K2797013

Designed by: Kyle Stanforth   Group: iGEM18_Newcastle   (2018-10-04)
Revision as of 11:37, 9 October 2018 by KyleStanforth (Talk | contribs) (→‎Usage and Biology)


High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard

pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter.

Usage and Biology

In summer 2018, [http://2018.igem.org/Team:Newcastle/InterLab Newcastle iGEM GeneMachine] designed a constitutive, medium strength RFP expression construct and cloned said construct into the backbone of the iGEM InterLab test device pSB1C3 vectors - in the non-coding region between the chloramphenicol resistance gene and the Origin of Replication - for use as an Internal Standard within the iGEM 2018 InterLab study.

The addition of this RFP Internal Standard within the InterLab study devices revealed that, even under the same experimental conditions, the complex nature of biological systems still cause variation in protein expression, and highlights how important the use of an Internal Standard is in the identification of variation in part characterisation studies.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 989
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 1905
    Illegal PstI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 989
    Illegal EcoRI site found at 3052
    Illegal NheI site found at 1017
    Illegal NheI site found at 1040
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1905
    Illegal PstI site found at 16
    Illegal PstI site found at 1919
    Illegal NotI site found at 9
    Illegal NotI site found at 995
    Illegal NotI site found at 1912
    Illegal NotI site found at 3058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 989
    Illegal EcoRI site found at 3052
    Illegal XhoI site found at 2036
    Illegal XhoI site found at 2928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 989
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 1905
    Illegal PstI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 989
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 1004
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1905
    Illegal PstI site found at 16
    Illegal PstI site found at 1919
    Illegal AgeI site found at 1627
    Illegal AgeI site found at 1739
  • 1000
    COMPATIBLE WITH RFC[1000]


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