DNA

Part:BBa_K2889003:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 06:39, 9 October 2018 by Ruizhou (Talk | contribs) (→‎Applications of BBa_K2889003)


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Applications of BBa_K2889003

In our previous project, we constructed two miR-21 sponge plasmid, pEGFP-miR-21-sponge-2s and pEGFP-miR-21-sponge-6s, as the monitor to detect the expression of miR-21 in cells.However, we’d like to ascertain a preferable binding sites number contained in miRNA sponge as a sensitivity and specificity monitor this year.Hence, we constructed pEGFP-miR-21-sponge-4s.We find that miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge6s.

The_effect_of_pEGFP-miR-21-sponge-4s_on_the_correlative_between_fluorescence_value_of_GFP_and_copy_numbers_of_miR-21.jpg

The_standard_curve_of_miR-21-sponge-4s.jpg

User Reviews

UNIQ18bab5b822247591-partinfo-00000000-QINU UNIQ18bab5b822247591-partinfo-00000001-QINU