Plasmid

Part:BBa_K2797013

Designed by: Kyle Stanforth   Group: iGEM18_Newcastle   (2018-10-04)
Revision as of 14:14, 4 October 2018 by Registry (Talk | contribs)

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High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard

pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 989
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 1905
    Illegal PstI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 989
    Illegal EcoRI site found at 3052
    Illegal NheI site found at 1017
    Illegal NheI site found at 1040
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1905
    Illegal PstI site found at 16
    Illegal PstI site found at 1919
    Illegal NotI site found at 9
    Illegal NotI site found at 995
    Illegal NotI site found at 1912
    Illegal NotI site found at 3058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 989
    Illegal EcoRI site found at 3052
    Illegal XhoI site found at 2036
    Illegal XhoI site found at 2928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 989
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 1905
    Illegal PstI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 989
    Illegal EcoRI site found at 3052
    Illegal XbaI site found at 1004
    Illegal XbaI site found at 3067
    Illegal SpeI site found at 2
    Illegal SpeI site found at 1905
    Illegal PstI site found at 16
    Illegal PstI site found at 1919
    Illegal AgeI site found at 1627
    Illegal AgeI site found at 1739
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None