DNA

Part:BBa_K2889003:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 06:35, 9 October 2018 by Ruizhou (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2889003

In our previous project, we constructed two miR-21 sponge plasmid, pEGFP-miR-21-sponge-2s and pEGFP-miR-21-sponge-6s, as the monitor to detect the expression of miR-21 in cells.However, we’d like to ascertain a preferable binding sites number contained in miRNA sponge as a sensitivity and specificity monitor this year.Hence, we constructed pEGFP-miR-21-sponge-4s.We find that miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge6s.

The_effect_of_pEGFP-miR-21-sponge-4s_on_the_correlative_between_fluorescence_value_of_GFP_and_copy_numbers_of_miR-21.jpg

User Reviews

UNIQ87dacd730a48cb06-partinfo-00000000-QINU UNIQ87dacd730a48cb06-partinfo-00000001-QINU