Coding

Part:BBa_K2631000

Designed by: Mian Xia   Group: iGEM18_CIEI-BJ   (2018-10-09)
Revision as of 05:49, 9 October 2018 by Mian (Talk | contribs)


ADTZ Enzyme

ADTZ, which is also called aflatoxin oxidase (AFO), is the first enzyme identified to bed able to degrade AFB1. It can be isolated from intracellular extracts and show a strong affinity with AFB1. As a potential degradation enzyme candidate in our project. In order to express soluble ADTZ in E．coli，the DNA coding ADTZ was inserted into the vector pMAL-c5x to produce the fusion protein of maltose-binding protein (MBP) and ADTZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1556
    Illegal BglII site found at 1979
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None