Composite

Part:BBa_K2804001:Design

Designed by: Byron Francisco Mosquera   Group: iGEM18_Ecuador   (2018-10-05)
Revision as of 05:47, 9 October 2018 by Pancho Mosquera (Talk | contribs) (→‎Design Notes)

Bone morphogenetic protein 2 (BMP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because the N-terminal domain of BMP2 has been mostly fused to proteins, we have decided to fuse it N-terminally to the CBD cipA.

Source

3A Assembly with CBD cipA, BMP2 as gBlocks and psb1c3 as the cloning vector.

References

McCarthy, B., Yuan, Y., & Koria, P. (2016). Elastin-like-polypeptide based fusion proteins for osteogenic factor delivery in bone healing. Biotechnology Progress, 32(4):1029-37.

Source

Synthetized from IDT.

References

Schmoekel, H.G., Weber, F.E., Schense, J.C., Grätz, K.W., Schawalder, P. & Hubbell, J.A. (2005). Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices. Biotechnology and Bioengineering; 89(3):253-62.