Part:BBa_K2543010
Ac5-hCD4-dToll-polyA / pSB1C3
Ac5 is a strong and constitutive promoter from Drosophila actin 5c gene and commonly used in insect expression system. Human CD4 (hCD4) is a cell marker expressed on the subtype of T helper cell. CD4 acts as a coreceptor to help T cell development and cell function.
CD4 plays an important role in T cell activation and immune signaling. The extracellular domain of hCD4 (1-396 aa) can form dimer and regulate the function of T cell activation.
Toll is a transmembrane protein involved in insect immune defense system to recognize pathogens like bacteria, viruses and fungi. Toll activated by pathogens transmits the signaling to express anti-microbial peptide (AMP) to kill the pathogens. Drosophila transmembrane domain (808-828 aa) and intracellular domain (829-1097 aa) of Toll (dToll) play an important roll in regulating the immune signaling.
Polyadenylation (polyA) is one of the process of eukaryotic mRNA translation. It adds a poly(A) tail to protect mRNA from enzymatic degradation and aid in transcription termination. Polyadenylation signal of simian virus 40 (SV40 poly A) has been used widely in mammalian and many eukaryotic gene expression system.
This construct creates a synthetic human CD4-drosophila Toll chimera receptor system. The system functions not only in response to human HIV virus and also transmit Toll signaling to activate the expression of anti-microbial peptide (AMP)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 104
Illegal XhoI site found at 3691
Illegal XhoI site found at 4153 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3760
Illegal NgoMIV site found at 5079
Illegal AgeI site found at 3742 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2923
Illegal BsaI site found at 4549
Illegal BsaI.rc site found at 2533
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