Part:BBa_K2570000

PAR

To optimize the engineered 2-PE biosynthetic pathway, the Par (BAG13450.2) the phenyla- cetaldehyde reductase from the rose (Rosa sp.)

Construction and optimization of the 2-PE biosynthetic pathway in E.coli.

A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 247
  Illegal NotI site found at 610
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]