RBS

Part:BBa_K2541301:Design

Designed by: Zihao Wang   Group: iGEM18_Jilin_China   (2018-10-05)
Revision as of 07:27, 8 October 2018 by Shuting (Talk | contribs) (→‎References)


Cold-inducible RNA-based thermosensor-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.


Source

The natural cspA gene sequence is from Escherichia coli. The sequence we used is the 5'UTR of cspA. And we incrase or decrease the base pairings and GC centent of this sequence. We synthesized this sequence from a synthesis company.

References

[1]Giuliodori A M, Di P F, Marzi S, et al. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.[J]. Molecular Cell, 2010, 37(1):21-33.

[2]Breaker R R. RNA Switches Out in the Cold[J]. Molecular Cell, 2010, 37(1):1-2.

[3]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.