RNA
Part:BBa_K2889002:Design
Designed by: Rui Zhou Group: iGEM18_Worldshaper-Wuhan (2018-10-04)
pSB1C3-IL7-AS-S1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 626
Illegal SpeI site found at 813
Illegal SpeI site found at 924 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 600
Illegal SpeI site found at 813
Illegal SpeI site found at 924 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to investigate which domain is essential for the function of IL7-AS. We cloned the truncated sequences of IL7-AS (IL7-AS-S1) into pSB1C3 for submitting to IGEM 2018 and inserted IL7-AS-S1 into pCDNA3.1 to study the function.
Source
We cloned the truncated sequences of IL7-AS (IL7-AS-S1, 1099 bp) from human cells