RNA

Part:BBa_K2889002:Design

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 09:33, 4 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pSB1C3-IL7-AS-S1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 626
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to investigate which domain is essential for the function of IL7-AS. We cloned the truncated sequences of IL7-AS (IL7-AS-S1) into pSB1C3 for submitting to IGEM 2018 and inserted IL7-AS-S1 into pCDNA3.1 to study the function.


Source

We cloned the truncated sequences of IL7-AS (IL7-AS-S1, 1099 bp) from human cells

References