RNA

Part:BBa_K2889002

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 09:33, 4 October 2018 by Registry (Talk | contribs)

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pSB1C3-IL7-AS-S1

We cloned the full length of IL7-AS, two truncated sequences of IL7-AS (IL7-AS-S1 and IL7-AS-S2) into PCDNA3.1 and transfected these plasmids to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells. However IL7-AS-S1 and IL7-AS-S2 did not influence the migration of 786-O cells, suggesting IL7-AS-S1 and IL7-AS-S2 lack of key structural domains.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 626
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 600
    Illegal SpeI site found at 813
    Illegal SpeI site found at 924
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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