Part:BBa_K2573000

MetE Coding Device

Usage and Biology

Methionine is an essential amino acid for E.coli growth. E. coli MetE codes for an enzyme that catalyzes the final step of de novo methionine biosynthesis without using an intermediate methyl carrier and in the absence of vitamin B12.

• IMAGE* https://www.researchgate.net/figure/Biosynthesis-of-methionine-A-Methionine-synthase-MetH-MetE-reaction-B_fig8_276507951

A 2.4kb fragment containing the MetE gene from plasmid pSK397 was cloned into bba_J4450 via PCR and NEBuild Hi-Fi Assembly.

CHARACTERIZATION: By introducing the MetE gene into plasmid desired for insert it can be used to differentiate between E.coli cells that have successfully taken up the insert and those that have not in a medium lacking methionine.

REFERENCES: Pejchal, Robert, and Martha L Ludwig. “Cobalamin-Independent Methionine Synthase (MetE): A Face-to-Face Double Barrel That Evolved by Gene Duplication.” PLOS ONE, Public Library of Science, journals.plos.org/plosbiology/article?id=10.1371%2Fjournal.pbio.0030031.

“Escherichia Coli K-12 Substr. MG1655 MetE.” MetaCyc Parathion Hydrolase, biocyc.org/gene?orgid=ECOLI&id=EG10584.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1864
  
• 1000
  COMPATIBLE WITH RFC[1000]