Coding
Part:BBa_K2665011
Designed by: Masahiro Nanbu Group: iGEM18_Kyoto (2018-09-25)
TDH3-Mangrin-6xHis-CYC
This part coded salt tolerance protein, Mangrin form mangrove. This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. Mangrin is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. We used this part for improving halotorelance of yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 842
Illegal BamHI site found at 936 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
For the confirmation of the mangrin function of mangrin, we spotted
Reference
[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910
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Categories
Parameters
None |