Conjugation

Part:BBa_I714031:Experience

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-21)
Revision as of 19:25, 21 October 2009 by Cplesa (Talk | contribs)

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Applications of BBa_I714031

Characterization of BBa_I714031

A [http://2009.igem.org/Team:TUDelft/ConjugationProtocol conjugation test] was done by the TUDelft iGEM 2009 team to characterize this part.

Donors: [http://www.cbs.knaw.nl/databases/nccb/search_bac_plas.aspx NCCBNr# 2350] cells with R751 and I13522+I714031-pSB4C5 (TRI+CAM)
Receivers: DH5 α cells with J23100 (AMP)
Transconjugants A: DH5 α cells with R751 and J23100 (TRI+AMP)
Transconjugants B: DH5 α cells with I13522+I714031-pSB4C5 and J23100 (CAM+AMP)

The following table shows the calculated conjugation efficiencies (raw data [http://2009.igem.org/Team:TUDelft/19_August_2009#Calin here]):

Set # Conjugation Efficiency for I13522+I714031-pSB4C5 Conjugation Efficiency for wild R751
1 0.00177 0.0141
2 0.00224 0.0476
3 n/a 0.0356


The suspected reason for the lower efficiency of I13522+I714031-pSB4C5 compared to R751 is the presence of the entry exclusion protein trbK. If a receiver cell gets I13522+I714031-pSB4C5, it can still receive R751 afterward. But if a receiver cell gets R751, it will not accept I13522+I714031-pSB4C5 afterward because trbK expression will block incoming transfers.

User Reviews

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  • For some data about the efficiency test of conjugation, please see the following link: [http://2007.igem.org/Peking_Hop-Count Data of Conjugation]