![](https://parts.igem.org/images/partbypart/icon_conjugation.png)
Conjugation
Part:BBa_I714031
Designed by: Mingzhi Qu Group: iGEM07_Peking (2007-10-21)
OriT-R (Origin of Transfer for the R751-plasmid nic region)
OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
Characterization
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 51
Illegal NgoMIV site found at 104
Illegal NgoMIV site found at 114 - 1000COMPATIBLE WITH RFC[1000]
// Using point mutation to obtain right sequence.
[edit]
Categories
Parameters
//DNA/conjugation
//function/conjugation
//function/conjugation
None |