Generator

Part:BBa_K2609017

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-24)
Revision as of 10:03, 24 September 2018 by Registry (Talk | contribs)

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T4 endolysin under a constitutive promoter

Expresses the T4 endolysion gene under BBa_J23106, a medium strength constitutive promoter. This part was used to screen for recombined phages after Lambda Red recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 311
    Illegal AgeI site found at 381
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None