Part:BBa_K2856001

Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)

The BBa_K2856001 harbors a coding sequence of bifunctional glutamate--cysteine ligase/glutathione synthase (gshF) derived from Streptococcus. Codon-optimization has been made for Lactococcus Lactis. gshFp catalyzes the conversion of Cys,Glu and Gly to GSH.

Usage and Biology

Bifunctional glutamate--cysteine ligase/glutathione synthase (gshF) is an enzyme involved and responded to synthetic reaction of GSH.In this reaction,one Cys and one Glu are converted to one γ-GC,then one γ-GC and one Gly are converted to one GSH.(Figure 1) The Lactococcus Lactis NZ9000 has inability to synthesis GSH.In our project,we construct a plasmid harboring gshF in order to produnce GSH in Lactococcus Lactis NZ9000.

Figure. 1 Enzymatic reaction catalyzed by gshF

Protein Analysis

Figure. 2 gshF protein analysis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]