Composite

Part:BBa_K2789003

Designed by: Du liaoqi   Group: iGEM18_WHU-China   (2018-10-05)
Revision as of 01:50, 6 October 2018 by Registry (Talk | contribs)

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lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator+cpcg+non gate+rbs+gfp+termiantor

DNA element : 1.Ccar and Ccas : They could form heterodimer as transcription factor to activate the Cpcg (light control promotor) under green light. And the heterodimer is unstable under blue light. 2.Lac operator : They could block the expression of downstream genes and regain the promotor ability after inducted by IPTG 3.Cpcg : The promotor could be activated by heterodimer made up of Ccar and Ccas 4.TetR : TetR could bind to the tetracydine operator tetO to inhibit transcription . 5.TetO : TetO could be inhibited by tetR


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
    Illegal BamHI site found at 2973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 1998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5242


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