Regulatory

Part:BBa_K2540010

Designed by: Anna Guseva   Group: iGEM18_Rice   (2018-10-05)
Revision as of 05:34, 5 October 2018 by Registry (Talk | contribs)

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Broad host range regulatory element (strength 10)

This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 29572) and is originally found in Eubacterium rectale.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 19
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 183
    Illegal SapI.rc site found at 171


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Parameters
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