Part:BBa_K2540003
Broad host range regulatory element (strength 8)
This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 36709) and is originally found in Acaryochloris marina.
Usage and Biology
The broad host range regulatory element was characterized by Johns et al. among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns et al. are given below:
Table 1: Relative transcription and translation levels for the broad host range element (strength 5).
The part was characterized in four E. coli strains, S. oneidensis , and P. putida using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB for 24 hours.
Figure 1: Fluorescence levels of mKate2 under the control of the broad host range element (strength 5).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 183
References
Johns, N. I. et al.(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.
| None |