RBS

Part:BBa_K2541002:Design

Designed by: Zihao Wang   Group: iGEM18_Jilin_China   (2018-09-30)
Revision as of 03:30, 5 October 2018 by Shuting (Talk | contribs) (→‎Design Notes)


Heat-inducible RNA-based thermosensor-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.

Source

Our construction device includes a promoter J23101 and chromoprotein tspurple, which can be replaced by the BbsI restriction endonuclease, cjblue that can be replaced by the BsaI restriction endonuclease, conserved sequence and reporter protein sfGFP. Such a composition can be realized that when both the replacement part I and the replacement part II are not replaced, the colony exhibits the color of the tspurple, and when the replacement part I is replaced with another promoter by using BbsI, the colony appears blue, and the unsuccessful colony appears Purple. When BsaI was used to replace Part II, the colony showed the original milky white color of the bacteria, and the blue color colony is unsuccess.

References