Part:BBa_K2541002:Design
Heat-inducible RNA-based thermosensor-2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Through the visible color changes, we can select successful colonies from the culture dish. In this way, we can eliminate the gel midi purification work, reduce the workload and increase the work efficiency compared with the traditional construction method. There is also a direction in the choice of picking up monoclonal colonies.
Source
Our construction device includes a promoter J23101 and chromoprotein tspurple, which can be replaced by the BbsI restriction endonuclease, cjblue that can be replaced by the BsaI restriction endonuclease, conserved sequence and reporter protein sfGFP. Such a composition can be realized that when both the replacement part I and the replacement part II are not replaced, the colony exhibits the color of the tspurple, and when the replacement part I is replaced with another promoter by using BbsI, the colony appears blue, and the unsuccessful colony appears Purple. When BsaI was used to replace Part II, the colony showed the original milky white color of the bacteria, and the blue color colony is unsuccess.