Composite

Part:BBa_K2593007

Designed by: Huimin Li   Group: iGEM18_SSTi-SZGD   (2018-09-21)
Revision as of 08:08, 21 September 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This part is a functional composite part of HasA synthetase production.Its consists of a promoter,


xylR+PxylA: A xylose inducible promoter with its transcriptional regulator. The xylose inducible promoter used to construct this part is amplified from the integration plasmid pAX01.. This part contains a xylose inducible promoter which is originated from Bacillis megaterium. The xylose inducible promoter we constructed contains promoter PxylA which located within xylose operon, originally to drive the expression of xylA (xylose isomerase coding gene) and xylB (xylulose kinase). xylR with its promoter located at upstream of xylose operon is also included in our parts. It encodes xyl repressor which binds to xyl operator in the absence of xylose, repressing transcription event. In the presence of glucose, glucose-6-phosphate metabolized from glucose can compete with xylose in the binding site of xylose on XylR. In addition, glucose itself is also supposed to be a low efficiency inducer for XylR. (DAHL, 1997) Therefore while xylose induces transcription, the existence of glucose, to some extent, represses gene transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1665


[edit]
Categories
Parameters
None