Part:BBa_K2555002

pBAD-RBS-CueR

This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct.CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 is used for construction of BBa_K2555004.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1311
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1250
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1085
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1067
  

References

(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.