Coding

Part:BBa_K2865001:Design

Designed by: Chuqi Wang   Group: iGEM18_SMMU-China   (2018-09-29)
Revision as of 13:31, 29 September 2018 by Registry (Talk | contribs)

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AR185-T2A-EGFP, nanobody inhibiting RyR2 phosphorylation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 155
    Illegal NgoMIV site found at 302
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RyR2-specific nanobodies were isolated from a phage display library of variable domains of camellidae heavy chain-only antibodies (VHH). AR185, one of the isolated nanobodies, inhibiting RyR2 phosphorylation in an in vitro assay was then chosen.


Source

This nanobody was acquired through phage display and biopanning.

References