Coding

Part:BBa_K2587009

Designed by: Susanne Vollmer   Group: iGEM18_Duesseldorf   (2018-09-18)
Revision as of 09:27, 2 October 2018 by Suvol101 (Talk | contribs)


ptxD_opt

ptxD is coding for the phosphonate dehydrogenase, that catalyses the oxidation of phosphite (phosphonate) using NAD+ and H20 to Phosphate and NADH. pH optimum is pH = 7.25-7.75.

Usage and Biology

Most of the microorganisms, espacially common used modelorgansims like Escherichia coli or Saccharomyces cerevisiae grow only very slow on phosphite as phosphorus source. As a result the ptxD gene together with a phosphite media could be used as a selection marker. Without a permanent usage of antibiotics a screening and a decrease of the contamination risk could be achieved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6
    Illegal BamHI site found at 1044
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI.rc site found at 1051


[edit]
Categories
//awards/basic_part/nominee
Parameters
None