Coding

Part:BBa_K2609005:Design

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-23)
Revision as of 05:42, 2 October 2018 by BhaskarK (Talk | contribs) (→‎Design Notes)


T4 bacteriophage long tail fiber protein gp37 (Modification IV)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____

A double stop codon is placed at the end to ensure proper termination.

Source

T4 bacteriophage genome, sequence from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/HM137666)

References