Plasmid_Backbone
pSB4K5

Part:pSB4K5

Designed by: Reshma Shetty   Group: iGEM06_Bangalore   (2007-02-28)
Revision as of 19:03, 28 February 2007 by Registry (Talk | contribs)

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Low copy BioBrick standard vector

pSB4K5 is a low copy plasmid with kanamycin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3398
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3398
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3398
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3398
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3413
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/low
//plasmidbackbone/operation
//plasmidbackbone/version/5
Parameters
chassis
copies~5
mcsBioBrick
originpSC101
resistanceK