Composite
CAHS 1

Part:BBa_K2623014

Designed by: Hongling Liu   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 18:52, 28 September 2018 by Registry (Talk | contribs)

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Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter(BBa_J23100) into the CAHS circuit to express Lac I(BBa_S0100) in order to inhabit the initiation of the Lac O(BBa_R0011) promoter which can only be activated and then expressing CAHS and mRFP(BBa_J04650) after IPTG is added. The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 375
    Illegal NheI site found at 1679
    Illegal NheI site found at 1702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1376
    Illegal AgeI site found at 1488
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None