Composite

Part:BBa_K2587024:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-30)
Revision as of 19:28, 30 September 2018 by Registry (Talk | contribs)

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luxI_luxR_Plux_E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 1681
    Illegal BsaI site found at 1832
    Illegal BsaI.rc site found at 1523
    Illegal BsaI.rc site found at 1790


Design Notes

The parts LuxI, LuxR, pLux and phiX174E are codon optimised for E.coli. The construct is assembled using the CIDAR MoClo toolbox and therefore contains Type II S restriction sites. It can therefore be further used for assemblies with the MoClo toolbox or with other parts containing the respective overhangs.


Source

The sequences for LuxI, LuxR, pLux and phiX174 E are from GenBank and codon optimised for E.coli. The remaining sequences are from the CIDAR MoClo toolbox.

References