Tag

Part:BBa_K2560257:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-29)
Revision as of 21:33, 29 September 2018 by Registry (Talk | contribs)

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4x Streptag for N-terminal protein tagging


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon.


Source

Source of the part:

The sequence of this part was used from Addgene (Plasmid #55181). To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon.

The part was codonoptimized for V. natriegens and then synthetisized by IDT and integrated into the vector BBa_K2560002 via BsmBI

References