Project

Part:BBa_K2865002

Designed by: Chuqi Wang   Group: iGEM18_SMMU-China   (2018-09-29)
Revision as of 15:13, 29 September 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


[AAV9]-Left-ITR

The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which was shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None