Coding

Part:BBa_K2762004:Design

Designed by: HUI YEE NG   Group: iGEM18_NCKU_Tainan   (2018-09-23)
Revision as of 18:32, 27 September 2018 by Huiyee11186 (Talk | contribs) (→‎Design Notes)


CcaA (formerly icfA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole genome sequence of Synechococcus sp. PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

https://www.ncbi.nlm.nih.gov/nuccore/169884305

References