Project

Part:BBa_K2555003

Designed by: Tingyue Li   Group: iGEM18_BFSUICC-China   (2018-09-20)
Revision as of 14:15, 20 September 2018 by Registry (Talk | contribs)

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pBAD-RBS-sfGFP

This is BBa_K2555003 which used to test value of CueR used in BBa_K2555003. The plasmid including araC, araBAD promoter, RBS, and superfolder GFP. When the Ara enter the part, it will combined with araC, which active araBAD combining with RBS to produce CueR. Then the super folder GFP will generate a certain amount of fluorescence, which can be easily observed. Through this way, we can know the amount of CueR generated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355


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Categories
Parameters
None