Coding

Part:BBa_K2560253:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 19:19, 28 September 2018 by Marchal (Talk | contribs) (→‎References)


birA from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the birA gene of C. glutamicum (NCgl0679) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

If birA shall be used with an 5' tag use BBa_K2560256, if there is no need for tagging, use this part.

GGTCTCGAATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.


Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0679

Accession number of encoded protein: NP_599941.1

birA was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI


References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765

Samols D., Thornton CH., Murtif VL., Kumar GK., Haase FC., Wood HG. (1988). Evolutionary conservation among biotin enzymes. J. Biol. Chem. 263. 6461-6464.