Coding

Part:BBa_K2560252

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 17:02, 28 September 2018 by Registry (Talk | contribs)

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accD subunit from C. glutamicum, codonoptimized for V. natriegens

accD encodes the carboxytransferase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA). For this part, accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI. If accD shall be used with an 5' tag use BBa_K2560255, if there is no need for tagging, use this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None