Coding

Part:BBa_K2656013

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-20)
Revision as of 10:06, 28 September 2018 by Adrireq (Talk | contribs)


sfGFP Coding Sequence

Part BBa_I746916 standarized as Phytobrick. Super Folder Green Fluorescent Protein. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.

We have obtained the emission and excitation spectrum of this protein following this protocol and with these parameters:

Parameter Value
Minimum excitation wavelenght 0
Maximum excitation wavelenght 0
Emission wavelenght 0
Minimum emission wavelenght 0
Maximun emission wavelenght 0
Excitation wavelenght 0


This is the spectrum of sfGFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
//test
Parameters
None