Coding

Part:BBa_K2762004

Designed by: HUI YEE NG   Group: iGEM18_NCKU_Tainan   (2018-09-23)
Revision as of 09:42, 23 September 2018 by Registry (Talk | contribs)

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CcaA (formerly icfA)

  • Usage and *Biology **
    This BioBrick is a cloning intermediate of the carboxysome from Synechococcus elongatus PCC7002. The carboxysomal carbonic anhydrase (CA) of Synechococcus elongatus PCC7002, CcaA is needed for the CO2 fixation in the working carboxysome as it converts incoming hydrogen carbonate into carbon dioxide inside of the carboxysome. This step is essential for the CO2 fixation since it can increases the intracellular CO2 concentration, indirectly affect the CO2 fixation rate.

     CcaA is one of the beta-class CA which can be found in plants, algae, bacteria, and archaea, and is far more diverse in sequence than the other two classes, with only five residues (three forming the zinc ligand) being completely conserved.  It is a zinc-containing enzyme that catalyzes the reversible hydration of carbon dioxide. It has a tertiary fold, with a central 10-stranded beta-sheet as the dominating secondary structure element. The zinc ion is located in a cone-shaped cavity and coordinated to three histidyl residues and a solvent molecule.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None