Coding

Part:BBa_K2586019:Design

Designed by: Robert Warneke, Janek Meiner   Group: iGEM18_Goettingen   (2018-09-11)
Revision as of 13:50, 11 September 2018 by Dschannek96 (Talk | contribs) (→‎References)


GAT: Glyphosate N-Acetyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted the ordered GAT-enzyme into the PstI and EcoRI digested pSB1C3 backbone


Source

This part was first described in: Castle et. al. paper (2004). We ordered the gene from IDT (Integrated DNA Technologies) and used it as insert for our experiments.

References

<a href="http://science.sciencemag.org/content/304/5674/1151">Castle et. al. 2004</a> (1) Castle et. al. 2004: Discovery and Directed Evolution of a Glyphosate Tolerance Gene