Translational_Unit
Csy4-F155A

Part:BBa_K2615006:Design

Designed by: Anyi Li   Group: iGEM18_OUC-China   (2018-09-07)
Revision as of 12:36, 11 September 2018 by Anyi (Talk | contribs) (→‎Design Notes)


Csy4-F155A, the No.4 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

We designed this part by point mutation. We changed the TTC(encoding Phe) to GCG(encoding Ara) on the 155th site based on wild type Csy4.

Source

From

References