Coding
Part:BBa_K2671337
Designed by: Oliver Hild Walett, Johan Larsson Group: iGEM18_Linkoping_Sweden (2018-09-11)
GroES
The gene coding for the chaperone GroES (homolog to HSP10 in eukaryotes) that is found in E.coli. Often works in conjunction to GroEL creating an environment well suited for the folding of proteins.
The gene coding for GroES is placed downstream from a tetracycline promotor. Further upstream the tetracycline repressor protein (TetR) is found, expressed by a constitutive promotor. The TetR protein has a termination sequence directly downstream from it, while the GroES gene has its transcription stop by the E.coli his operon termination sequence that is present in most pSB vectors, directly downstream of the insert.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |