Coding

Part:BBa_K2586001:Design

Designed by: Robert Warneke, Janek Meißner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 06:47, 11 September 2018 by FMCommmichau (Talk | contribs)


GltT: glutamate and glyphosate transporter in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain B. subtilis 168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).

References