Intermediate

Part:BBa_I759002:Design

Designed by: Josh Michener   Group: iGEM07_Caltech   (2007-06-27)
Revision as of 04:05, 27 October 2007 by Cal (Talk | contribs) (→‎Source)


pTet-spacer-cro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in.

Source

Ptet was a standardized iGEM part, and the cro gene was from the lambda zap genome. Spacer-primers were specially designed so that the two parts (pTet and cro gene) could, through overlap extension PCR, be joined by the intermediary spacer.

References